High Performance Liquid Chromatography (HPLC) is an analytical technique for separating organic compounds in order to analyze their properties. HPLC is utilized in many industries such as biological, pharmaceutical, food, environmental and cannabis to analyze samples.
HPLC is a very popular instrument for compound analysis since it is simple to learn and easy to use. Modern HPLC are applied in quality control (QC) and research and development (R&D) to separate, identify, purify and quantify compounds. In the pharmaceutical industry, it is commonly used to quantify the active pharmaceutical ingredient (API) or label claim (LC). In the cannabis industry, HPLC is the primary instrument to determine %THC, %THCA, %CBD and %CBDA.
- Introduction to High Performance Liquid Chromatography (HPLC) principles
- Preparation of mobile phase, samples and standards
- Perform system suitability analysis
- Column setup and troubleshooting
- HPLC identification, assay and impurities tests
- Operations of Agilent and Waters HPLC system
- Navigation through Millennium (Empower) and ChemStation software
- Introduction to method validation and technology transfer
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